Strenuous exercises greatly increase oxygen consumption in the whole body, especially in skeletal muscles. Large part of oxygen consumption is reduced to H2O and ATP, but smaller part (2-5%) results in an increased leakage of electrons from the mitochondrial respiratory chain, forming various reactive oxygen species-ROS (02, H2O,iOH). These free ra- dicals are capable of triggering a chain of damaging biochemical and physiological reactions (oxidative stress, lipid peroxi- dation), as a base for skeletal muscles damage after exercise. MDA (malondialdehyde) is a marker of exercise induced lipid peroxidation process. L-ascorbic acid is a major aqueous-phase antioxidant. To estimate antioxidant role of ascorbic acid we use rate between dehydroascorbate and ascorbate. In this paper those markers of lipid peroxidation (MDA, MDA ind, % ind MDA), and ascorbic acid status (ascorbic acid Asc, ascorbat A, dehidroascorbat DHA, DHA/A) were determinated in 30 stu- dents, in rest and after treadmill running protocol (Bruce Treadmill Protocol). It was found that after the treadmill test, plas- ma MDA level had increased from 3,04 to 4,39 M/L (p<0,0001) and % ind MDA decreased significantly from 35% to 24,7% (p=0,0003). Plasma ascorbic acid was also found to be higher after the treadmill test comparing to rest level (from 55,4 to 67,6 μM/L). DHA/A level in rest was 1,62 and after treadmill test it increased to 2,05 (p=0,0014). These results suggests that strenuous exercise increased process of lipid peroxidation (MDA †, % ind MDA), but in the same time incre- ased ascorbic acid level in plasma and DHA/A rate indicates stronger activation of nonenzymatic antioxidant defense system.
References
1.
Carr CA, Frei B. Toward a new recommended dietary allowance for vitamin C based on antioxidant and health effects in humans. Am J Clin Nutr. 69(6):1086–107.
2.
Alessio HM, Goldfarb AH, Cutler RG. MDA content increases in fast- and slow-twitch skeletal muscle with intensity of exercise in a rat. Am J Physiol Cell Physiol. 255(6).
3.
Venditti P, Meo S. Antioxidants. Tissue Damage, and Endurance in Trained and Untrained Young Male Rats. Arch Biochem Biophys. 331(1):63–8.
4.
Koska J, Blazicek P, Marko M, Grna JD, Kvetnansky R, Vigas M. Insulin; catecholamines; glucose and antioxidant enzymes in oxidative damage during different loads in healthy humans. Physiol Res. 49 Suppl 1.
5.
Miyazaki H, Oh-ishi S, Ookawara T, Kizaki T, Toshinai K, Ha S, et al. Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. Eur J Appl Physiol. 84(1–2):1–6.
6.
Davies KJA, Quintanilha AT, Brooks GA, Packer L. Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun. 107(4):1198–205.
7.
Halliwell B, Gutteridge JM. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys. 246(2):501–14.
8.
Miriæ D. Prouèavanje slobodnih radikala i antioksidantne zaštite u krvi i likvoru obolelih od meningitisa. Doktorska Disertacija.
9.
Lj P. Uticaj askorbinske kiseline na antioksidantni status u indukovanim stresnim stanjima. Doktorska Disertacija.
10.
Slater TF. Free-radical mechanisms in tissue injury. Biochem J. 222(1):1–15.
11.
Kaminski M, Boal R. An effect of ascorbic acid on delayed-onset muscle soreness. Pain. 50(3):317–21.
12.
Khassaf M, McArdle A, Esanu C, Vasilaki A, McArdle F, Griffiths RD, et al. Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. Physiol. 549(2):645–52.
13.
Tauler P, Aguiló A, Gimeno I, Noguera A, Agustí A, Tur JA, et al. Differential Response of Lymphocytes and Neutrophils to High Intensity Physical Activity and to Vitamin C Diet Supplementation. Free Radic Res. 37(9):931–8.
14.
Gleeson M, Robertson JD, Maughan RJ. Influence of exercise on ascorbic acid status in man. Clin Sci. 73:501–5.
15.
Duthie GG, Robertson JD, Maughan RJ, Morrice PC. Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Arch Biochem Biophys. 282(1):78–83.
16.
Rokitzki L, Logemann E, Sagredos AN, Murphy M, Wetzel-Roth W, Keul J. Lipid peroxidation and antioxidative vitamins under extreme endurance stress. Acta Physiol Scand. 151(2):149–58.
17.
Mastaloudis A, Morrow JD, Hopkins DW, Devaraj S, Traber MG. Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radic Biol Med. 36(10):1329–41.
18.
Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, et al. Plasma antioxidant status and cell injury after severe physical exercise. Proc Natl Acad Sci U S A. 100(9):5119–23.
19.
Dillard CJ, R.E. L, Savin WM, Dumelin EE, Tappel AL. Effects of exercise, vitamin E, and ozone on pulmonary function and lipid peroxidation. J Appl Physiol. 45:927–32.
20.
Pauling L. Živeti duže oseæati se bolje.
21.
S.B B. Farmakologija: opšti deo specijalni deo receptura.
22.
Howald H, Segesser B, Korner WF. Ascorbic acid and athletic performance. Ann N Y Acad Sci. 258:458–64.
23.
Nishikimi M, Fukuyama R, Minoshima S, Shimizu N, Yagi K. Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man. J Biol Chem. 269(18):13685–8.
24.
Bode AM, Yavarow CR, Fry DA, Vargas T. Enzymatic basis for altered ascorbic acid and dehydroascorbic acid levels in diabetes. Biochem Biophys Res Commun. 191(3):1347–53.
25.
Somogyi A, Szaleczky E, Pusztai P, Braun L, Sármán B. Plasma antioxidants in type 2 diabetes. Magy Belorv Arch. 50:187–92.
26.
Halliwell B. Drug antioxidant effects. A basis for drug selection? Drugs. 42:569–605.
27.
Sahnoun Z, Jamoussi K, Zeghal KM. Free radicals and antioxidants, human physiology pathology and therapeutic aspects (Article in French. Therapie. 52(4):251–70.
28.
Niš PSV.
29.
Ji LL. Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med. 18(6):1079–86.
30.
Sports Medicine AC. Guidelines for Exercise Testing and Prescription.
31.
Powers SK, Howley ET. Exercise Physiology: Theory and Application to Fitness and Performance.
32.
Baskin CR, Hinchcliff KW, DiSilvestro RA, Reinhart GA, Hayek MG, Chew BP, et al. Effects of dietary antioxidant supplementation on oxidative damage and resistance to oxidative damage during prolonged exercise in sled dogs. Am J Vet Res. 61(8):886–91.
33.
Ramel A, Wagner KH, Elmadfa I. Plasma antioxidants and lipid oxidation after submaximal resistance exercise in men. Eur J Nutr. 43(1):2–6.
34.
Lj P, RN M, M M, I R. Mehanizmi nastanka oksidativnog stresa izazvanog intenzivnom fizièkom aktivnošæu. Praxis Medica. 36(1–2):89–93.
35.
Groussard C, Rannou-Bekono F, Machefer G, Chevanne M, Vincent S, Sergent O, et al. Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. Eur J Appl Physiol. 89(1):14–20.
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