Parodontopatija predstavlja inflamantornu reakciju na gram-negativne anaerobne bakterijske infektivne agense koja pogađa potporni aparat zuba, uključujući gingivu, parodontalni ligament, cement i alveolarnu kost. Cilj istraživanja bio je da se utvrdi kvantitativno-kvalitativni sastav mokroorganizama subgingivalnog plaka kod pacijenata obolelih od hronične i agresivne parodontopatije PCR metodom (eng. Polimerase Chain Reaction), a zatim proceni povezanost različitih stepena inflamacije i destrukcije tkiva parodoncijuma sa prisustvom i koncetracijom ovih mikroorganizama. Ispitivanjem je obuhvaćeno 70 pacijenata starosti od 16 do 65 godina. Utvrđivanje mikroorganizama u subgingivalnom plaku, određen je PCR metodom. U cilju postavljanja dijagnoze i određivanja stepena destrukcije parodontalnog tkiva, bili su korišćeni standardni epidemiološki kriterijumi: plak indeks (Silness-Löe), gingivalni indeks (Lӧe-Silness), SBI indeks (Mühleman-Son) i PDDZ. Prisustvo parodontopatogena u subgingivalnom plaku pokazalo je statističku vezu sa kliničkim parametrima težine parodontopatije i inflamacije gingive. Nalaz Porphyromonas gingivalis i Actinobacillus actinomycetemcomitans je bio statistički značajno češći kod pacijenata sa srednje teškom i teškom formom parodontopatije u odnosu na prosečnu dubinu parodontalnih džepova. Detekcija P.g. i A.a. je bila statistički značajno češća kod osoba sa umerenom i intezivnom inflamacijom gingive. Kvantitativno-kvalitativni sastav mikroorganizama subgingivalnog plaka ima direktan uticaj na inflamatorno-destruktivne procese u parodontalnim tkivima.
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