It is well known that intensive physical activity increase the production of reactive species of O and N (RONS), but 2 it is also known that moderate and regular physical activity has influence on antioxidative system adaptation and help in repairing process of oxidative damage. In this paper we estimated markers of lipid peroxidation (MDA, % ind MDA), and antioxidant status (Asc, DHA/A, GSH, CAT, Pex) for 30 students (control group), and 30 professional football players (regular intensive physical activity). In the group of professional sportsman there were significant increase of lipid peroxidation markers (MDA ↑, % ind MDA ↑), which was also followed by an increased antioxidant activity (Asc ↑, DHA/A ↑, GSH ↑, Pex ↑, CAT ≈). We can conclude that chronic physical activity significantly improves antioxidant status using the adaptation process.

Oxidative stress and associated oxidative damage are mediators of vascular injury and inflammation in many cardiovascular diseases, including hypertension, atherosclerosis, ischemic heart disease, diabetes. Xanthine oxidoreductase is one of the enzymes producing free radicals in the cardiovascular system, and it can contribute to the increment of the oxidative stress and, consequently, blood pressure. The xanthine oxidase is a molybdoenzyme capable of catalyzing the oxidation of hypoxanthine and xanthine in the process of purine metabolism. Xanthine oxidase can exist in two interconvertible forms, either as xanthine dehydrogenase or xanthine oxidase. The aim of the paper was investigate the activity xanthine oxidoreductase in the plasma patients with age related cataract, with hypertensive, and with heart failure. Clinical and biochemical researches were carried out in 73 patients with age related cataract, mean age 72±7 years, divided into group with hypertensive (N=35), with heart failure (N=22) and group normotensive and without heart failure (N=16). Plasma xanthine oxidoreductase activity was determined spectrophotometrically by measuring the formation of uric acid from hypoxanthine at 293 nm. The concentration of triglycerides (TG), total cholesterol (TC), LDL-C, HDL-C in plasma were determined. Our findings show a significantly increased activity xanthine oxidoreductase in plasma hypertensive patients (XO 9.18±1.5 U/L) (p< 0.001) and patients with heart failure (XO 10.44±1.53U/L) (p< 0.001), compared with values plasma xanthine oxidoreductase activity in normotensive and patients without heart failure (XOD 6.02±1.4 U/L). Concentrations of plasma TG, TC and LDL-C were elevated in groups with hypertensive and with heart failure, and we to find significant differences with group normotensive and without heart failure values. Elevated activity of plasma xanthine oxidoreductase contribute to the increasåd oxidative stress and may play a role in accelerated atherosclerosis. 

Metformin is an anti-hyperglycemic agent used for the treatment of type 2 diabetes mellitus. Biguanides lower blood glucose levels primarily by decreasing the amount of glucose produced by the liver. Metformin also helps to lower blood glucose levels by making muscle tissue more sensitive to insulin so glucose can be absorbe. The purpose of the study was to establish the effect of metformin on insulin secretion in obese patients with newly diagnosed diabetes mellitus type 2. We included 40 patients with Type-2 Diabetes Mellitus, who were treated on Department for Internal Disease in Hospital King Milutin Laplje Selo. Patients included in the study were newly diagnosed with diabetes mellitus type 2, glycemia < 12 mmol/L, and hed the Body Mass Index >30 kg/m2. They were treated with metformin, 1000 mg/day. Insulin sensitivity was determined by HOMA IR, insulin secretion by HOMA, lipid status by spectrophotometry. After six months of treatment blood biochemistry tested again. BMI was also established. Metformin significantly lowered BMI (BMI before therapy 31.02 kg/m2, after treatment 28.7 kg/m2) (both p<0,001 vs baseline) waist circumference mean value 98±0.55 cm-93,12 cm. (p<0,001) During the therapy the glycaemia decreased from 11.7 mmol - 6,78 mmol/l, insulinemia mean value 20.141 mlU/l -13.691, HOMA IR elevated in all patients. Among the cholesterol fractions, most significant results were obtained in LDL cholesterol. Mean LDL cholesterol levels 4.51± 2.02 mmol/L. Mean cholesterol level 6.98±2.31 mmol/L. HDL levels -1.32 mmol/L. Serum triglycerides was (mean) 2.63±1.32mmol/L. After six months therapy Metformin produces beneficial changes in lipid states. Metformin may be the first therapeutic option in the diabetes mellitus type 2 with overweight or obesity. Metformin produces beneficial changes in glycemia control, and moderated in weight, lipids and insulinemia. 

The discovery of Helicobacter pylori (Hp) and the causal relationship between Helicobacter pylori infection with chronic gastritis, gastric and duodenal ulcers and gastric cancer is an epochal event in recent times. The aim of this study was to examine the prevalence of Hp infection and prove the connection between Helicobacter pylori infection and gastric and duodenal diseases in different age and gender categories. Study included 250 subjects of both sexes, different age groups selected random sample. All respondents were subjected to Hp urease test from biopsy specimens of the antrum and corpus of the stomach obtained by upper gastrointestinal endoscopy with standard forceps. The data were analyzed using descriptive statistical methods (CF, SD, Min, Max) and analytical statistical methods. (t test, Chi square test, Kruskal-Wallis test, MannWhitney U test, Wilcoxon test, analysis of variance, ANOVA, Spearman correlation and Persona). Non ulcer gastropathy was the dominant finding that the Hp urease negative (92.2%) and the Hp urease positive (77%)respondents .Duodenalni ulcer was common in the male sex (24.6%) and gastric cancer (2.9%), and stomach ulcer was common in women (4.5%). The most common representation non ulcer gastropathy in the fifth and sixth decades of life while duodenal ulcer was most common in the fifth decade (37.5%). Gastric ulcer was noted in the second, third and fourth decade of life. Gastric cancer is 100% of cases was recorded in the sixth decade of life. Hp Urease test that requires upper digestive endoscopy and biopsy specimens of gastric mucosa is the minimum invasive diagnosis of Helicobacter pylori infection. Upper gastrointestinal endoscopy and HP verification changes the standard in the diagnosis of associated diseases of the stomach and duodenum. (non ulcer gastropathy, gastric and duodenal ulcers and gastric cancer).

Formation of vascular access in elderly and diabetic patients is burdened with many difficulties. The aim of this study was to determine the factors that older people and patients with diabetes mellitus defined as the population risk for the formation of arteriovenous fistula for hemodialysis. The study was organized as a retrospective study, a subject of interest in people older than 65 years of age and diabetes, which in four years because of the need for further treatment performed arteriovenous fistula. Analyzed the demographic structure of the patient, type of anastomosis, the use of central-venous catheters for hemodialysis, arterial blood pressure and size of the lumen of blood vessels used for anastomosis, intraoperative measured. From the laboratory analysis determined the routine hematological and biochemical parameters. In relation to the positioning of arteriovenous fistula (p=0.033), the placement of catheters for hemodialysis (p=0.0009), preventive form of vascular access (p=0.04) and values of systolic (p=0.0001) and diastolic (p=0.0001) arterial blood pressure, achieved a statistically significant difference. Patients with diabetic nephropathy, compared to the population of elderly, have a significantly higher number of leukocytes (7.5±2.61 vs. 8.5±3.24, p=0.03), fibrinogen level (5.57±2.39 vs. 6.8±1.71, p=0.036), glycemia (5.35±1.24 vs. 7.1±2.28, p<0.0001), cholesterol (3.77±1 , 03 vs. 4.5±1.71, p=0.001), triglycerides (1.59 ±0.62 vs. 1.86±0.76, p=0.009) and LDL-cholesterol (2.13±0.76 vs. 2.42±2.04, p=0.02). Total proteins (64±9.98 vs. 62 ±7.4, p=0.027) and albumin (36±34 vs. 9.55±5.49, p=0.037) were significantly lower values in diabetics. Characteristics of older diabetics and is characterized as a risk population of patients for the creation of arterio-fistula were timely creation and position of arterio fistula, the need for placement of central-venous catheters for hemodialysis and arterial blood pressure.

Hydropneumothorax is a patological condition of pleura characterised by presence of both liquid and air, rarely of gas between two pleura laminae.According to the origin,it can be result of a disease or a trauma. A typical roentgenogram is obtained during radiography in a standing position and PA projection: horizontal level of liquid represented by homogeneous shadow merging with diaphragm shadow and avascular light without visible parenchyma above the liquid level. The authors present two cases of hydropneumothorax. The first case is about hydropneumothorax originated after several repeated tapping of pleural effusion of malignant etiology. In the second case, hydropneumothorax is a thorax injury complication. Hydropneumothorax rarely appears in practice, and diagnosis is fast and accurate just based on clinical picture and standard lung radiography.

Diagnosis of different pathohystological diseases of prostate in the most cases based on common benignant and malignant characteristics. The presence of periacinar cleftings (PC) is an additional criterion favouring prostatic adenocarcinoma. The aim of our work was to examine the presence of PC around glands in prostatic adenocarcinoma (PA), prostatic intraepithelial neoplasia (PIN) and benign hyperplasia of prostate (BHP) and to determinate specificity and sensitiveness for their presence in PA. We analysed biopsy material of Institute of pathology, Medical faculty Priština and Department of pathology and forensic medicine Clinical center of Kragujevac from begining of 2007. till the end of 2008. According to the presence and extent of PC, analysed on high power field (400x), glands were classified as: group 1 - glands without PC or with PC affecting ≤50% of gland circumference; group 2 - glands with PC affecting >50% gland circumference in <50% examined glands and group 3 - glands with PC affecting >50% gland circumference in ≥50% examined glands. By the analyse of our material we found PC around glands in PA, PIN and BHP: the most glands in PA were group 2 (34 or 48,6%) and group 3 (31 or 44,3%), in PIN group 1 (12 or 60%) and group 2 (8 or 40%), in BHP glands at all 100% cases were group 1. We found sensitiveness 92,9% and specificity 73,3% for glands with PC at prostatic adenocarcinoma, which indicate that periacinar cleftings represent a reliable criterion in diagnosis prostatic adenocarcinoma. 

Hyperprolactinemia is the most common hypothalamic-pituitary axial disorder which is found in clinical practice. Increased level of prolactin, regardless of the cause, results in disorder of gonadal function and infertility, while normalization of the level of prolactin is associated with establishment of normal gonadal function and fertility. Establishment of the correct diagnosis is a great significance, because it is possible to normalize the level of prolactin and establish normal function of ovaries in more than 90% cases with specific therapy. The objective of the work is to study concentration of prolactin in each phase of menstrual cycle, its impact (influence) on FSH, LH and E2, as well as the influence of hyperprolactinemia on the rhythm of menstrual cycle. The study has encompassed 150 of women in reproductive period, from 18-40 years of age. The control group includes 50 of women, and there are 100 of women with menstrual cycle disorders (oligomenorrhea, polymenorrhea and secondary amenorrhea) which causes hyperprolactinemia. The turnout of hyperprolactinemia in causes the disorders of menstrual cycle (oligomenorrhea, polymenorrhea, amenorrhea) is 83%, on the basis of the results, wich demonstrates a serious problem and justifies a scientific study.

Doppler ultrasound check-up significantly increases a possibility of initial functioning of arteriovenous fistula. The aim of the study was the preoperative evaluation of vascular system of the extremity for creation of arteriovenous fistula for haemodialysis. The examination was organized at Urology and Nephrology Clinic, Clinical Centre "Kragujevac". The examined subjects were 87 patients, 52 (59,8%) male and 35 (40,2%) female, mean age of 61±11,1 years. There were performed the routine biochemical analyses, demographical structure and clinical characteristics (type of anastomosis, fistula placement, arterial blood pressure, lumen size of the artery and vein, measured intraoperatively by Doppler technique). In 72,1% of patients with functioning fistula there was made a terminal-lateral anastomosis, compared to 57,7% of patients with initial non-functioning fistula, there was found a statistically significant difference between the groups p=0,008. Radiocephalic fistula was created in 62,3% of the examined patients with initial functioning , in patients with non-functioning, the distal fistula was created in 38,5% of the examined patients. Regarding the type of the fistula location there was obtained statistically significant difference; p=0,04. There was found a statistically significant difference between the group of patients with initial functioning of arteriovenous and the group without, concerning the intraoperatively measured lumen of the cephalic vein (2±0,29 vs. 2±0,38 mm); p=0,03 (2,1±0,5 vs. 1,8±0,4); p=0,0009. Statistical significance between the groups was also found in the lumen of the cephalic vein verified by the ultrasound (2,1±0,53 vs. 1,8±0,25 mm); p=0,038. Peak of the systolic speed of the radial, corelated to the group of patients with and the group without initial functioning fistula (50 ± 12,42 vs. 40 ± 6,9 cm/sec); (p=0,0026), has statistically significant value. By the correlation of the group of patients with initial functioning and the group of patients without initial functioning, regarding hemoglobin (97 ± 16,1 vs. 88 ± 18,3 g/l); p=0,006 and the level of urea (22,6 ± 12,7 vs. 23,9 ± 9,8); p=0,02, there was found a statistically significant difference. Color Doppler ultrasound has an important role in preoperative evaluation of blood vessels and it represents a meaningful predictive parameter of functioning of arteriovenous fistula. 

Epstein-Barrov virus (EBV) is a cause of heterophile antibody positive infectious mononucleosis (IM),which is characterized by fever, sore throat, limfadenopatiom and atypical limfocitozom. IM is mainly a self-limited disease. Complications of the central nervous system, hepatitis (which can be fulminant), (splenomegalia, obstruction of the upper respiratory tract with bacterial can sometimes be deadly.