Introduction: Direct and indirect pulp capping is a therapeutic procedure to preserve the tooth's vitality. The aim was to scan electron and polarization microscopy: analyze changes in cellular and extracellular components of dental pulp after direct and indirect overlay with glass-ionomer cement (GIC), determine the appearance of the dentine surface after direct and indirect pulping overlap with GIC and forms a proposal for a clinical application of preparations if it provides good and effective dentingenesis. Methods: The experiment was performed on experimental animals (domestic swine). For this research, alkaline cement was used ALFAGAL* bejz. On the teeth of the experimental group, preparations of the V class were made from the vestibular side. Twenty-two teeth were made indirectly, and twenty –two teeth directly covered with pulp. After completion of the preparation, dentin is directly covered with glass-ionomer cement (GIC) Alfagal* bejz. Through the ALFAGAL material for definitively closing cavities (amalgam) is laid. On the teeth where the perforation of the pulp chamber was made, ALFAGAL bejz was loaded directly into the pulp chamber, and through this the cavity was definitely sealed with GIC FUJI IX (GC Japan). Suitably prepared teeth and pulp were observed by SEM and a polarization microscope. Results: By observing and analyzing the obtained results, on a polarization and scanning electron microscope compared to the control group, results were obtained which can have a significant clinical implication in the biological treatment of the pulp. Alfagalbejz is directly applied in the pulp chamber: the boundary between newly developed reparative dentine is detected in the normal dentine structure, amorphous dentine, reproduction of connective tissue cells without pro inflammatory reactions and initial dedifferentiation in odontoblast, young blood vessels and cells (fibrocytes, histiocytes and fibroblasts) are observed. Indirectly applied Alfagal* bejz indicates the presence of different maturation stages of dentin, dentinal canals are clearly visible with a normal dentin structure, zero amorphous dentine and immature dentine structure are observed as well. The presence of an amorphous calcite of the dentine structure indicates the initial creation of a solid tissue barrier, which is the goal of the experiment. Conclusion: The results obtained by experimental research suggest ALFAGAL bejz as a good material for the direct and indirect overlap of the pulp.

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