Reduced glutathion (GSH), glutathion-reductase (GR) and glutathion-peroxidase (GSH-Px) are significant indicators of oxidative stress. Oxidative stress is a consequence of increased production of reactive oxygen radicals and/or on the other hand, insufficient antioxidative protection. It is well-known that free radicals have key role in the large number of biological phenomena, such as differentiation, transformation, signal transduction, aging etc., and they are important factors in pathogenesis of various diseases (carcinoma, diabetes, Alzheimer, Parkinson, aterosclerosis). This paper presents results of investigation of content of GSH and activities of GR and GSH-Px in healthy patients and diabetes insulindependent patients between 20 and 75 years of age in the region of Indjija. Correlation between investigated parameters in healthy persons and diabetics was found, as well as with results of similar investigations in other countries. Investigated sample comprised 200 healthy patients (20-75 years) divided into 5 groups, and 80 diabetes patients divided into 4 groups. Every group consisted of equal number of males and females. Above mentioned parameters were determined in hemolysate. Parameters change depending on patient age, and changes are more intense in all patients. Some differences exist in correlation of our results with results obtained in other regions.
Ingraham LL, Meyer DL. Biochemistry of Dioxygen, Superoxide Ions, Biochemistry of the Elements. Vol. 4.
3.
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine.
4.
Kehrer JP. Free Radicals as Mediators of Tissue Injury and Disease. Vol. 23, Crit. Rev. Toxicol. p. 21–48.
5.
Baynes JW. Role of oxidative stress in development of complications in diabetes. Vol. 40, Diabetes. p. 405–40.
6.
Fritsch B, Dieckmann A, Menz B, Wendel A, Hempelmann E, Fritsch KG, et al. Oxygen free radicals and the Tissue Injury. In: Akademia Kiado. p. 397–406.
7.
Powers SK, Hamilton K. Antioxidants and Exercise. Vol. 18, Clinic in Sports Medicine. p. 525–35.
8.
In: Proceedings of the 16th Conference of the German Society of Biological Chemistry.
9.
Beutler E. Glutathione Reductase, Glutathione. In: Proceedings of the 16th Conference of the German Society of Biological Chemistry. p. 109–13.
10.
Chiu DT, Stults FH, Tappel AL. Purification and properties of rat lung soluble glutathione peroxidase. Vol. 445, Biochemica Biophysica Acta. p. 558–66.
11.
Majkić-Singh NT. Medicinska biohemija. Vol. 6.
12.
Duthie GG, Robertson JD, Maughan RJ. Morris, P.C.: Blood Antioxidant Status and Erythrocyte Lipid Peroxidation following Distance Running. Vol. 282, Arch. of Bioch. and Biophys. p. 78–83.
13.
Jozwiak Z, Jasnowska B. Changes in oxygen-metabolising enzymes and lipid peroxidation in human erythrocytes as a function of age of donor. Vol. 32, Mechanisms of Aging and Development. p. 77–83.
14.
Scott MD, Eaton JW, Kuypers FA, Chiu DT, Lubin BH. Enhancement of Erythrocyte Superoxide Dismutase Activity: Effects on Cellular Oxidant Defence. Vol. 74, Blood. p. 2542–9.
15.
Tolmasoff JM, Ono T, Cutler RG. Superoxide dismutase: correlation with lifespan and specific metabolic rate in primate species, Proc. Vol. 77, Natl. Acad. Sci. USA. p. 2777.
16.
Dominguez C, Ruiz E, Gussinye M, Carrascosa A. Oxidative stress at onset and in early stages of type I diabetes in children and adolescents. Vol. 21, Diabetes Care. p. 1736–42.
17.
Ruiz C, Alegria A, Barbera R, Farre R, Lagarda MJ. Lipid peroxidation and antioxidant enzyme activities in patients with type I diabetes mellitus. Vol. 59, Scand J. Clin. Lab. Invest. p. 99–105.
18.
Kedziora KZ, Luciak M, Blaszczyk J, Pawlak N. Lipid peroxidation and activities of antioxidant enzymes in erythrocytes of patients with non-insulin-dependent diabetes with or without diabetic nephropathy, Nephrol. Vol. 13. p. 2829–32.
19.
Ciuchi E, Odetti P, Praudo R. Relationship between glutathione and sorbitol concentrations in erythrocytes from diabetic patients. Vol. 45, Metabolism. p. 611–3.
20.
Stohs SJ, El-Rashidy EH, Lawson T, Kobayashi RH, Wulf BG, Potter JF. Changes in glutathione and glutathione metabolizing enzymes in human erythrocytes and lymphocytes as a function of age donor. Vol. 7.
21.
Atalay M, Laaksonen DE, Niskanen L, Uusitupa M, Hanninen O, Sen SK. Altered antioxidant enzyme defenses in insulin dependent diabetic men with increased resting and exercise-induced oxidative stress. Vol. 161, Acta Physiol. Scand. p. 195–201.
22.
B.V. Ć, G. GL, A. JG, D. P, T. C, M. P, et al. Selenium-Dependent and Selenium-Non-Dependent Glutathione Peroxidase in Patients with Balkan Endemic Nephropathy. Vol. 17, J. Envir. Path. Tox. One. p. 321–4.
23.
Cutler RG. Antioxidants and longevity of mammalian species, Molecular Biology of Aging.
24.
G. GL, V. Ć, A. JG, N. L, V Ć. Spasić M.: Selenium-Dependent and Selenium-Non-Dependent Glutathione Peroxidase in Patients with Balkan Endemic Nephropathy. Vol. 17, J. Envir. Path. Tox. One. p. 321–4.
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.
