Our methodological procedure established successful technique of the direct dyeing of elastic fibers and lamellas in elastic tissues. Experiments were carried out on a human autopsy material. Blocks rich in elastic fibers were fixed by 10% buffered neutral formaline and cut in the thickness of 5m. Deparafining of slides is done by xylol treatment (2x15min). Additional denaturation is achieved by combined solution, which contains 1.2g of picric acid in 30% solution of glacial acetic acid. By chloramine B application as blocking agent in form of 1% DMSO solution the dyes affinity for eviromental tissue is decreased. Tissues prepared in this way are treated with 0.5% solution of acidic sulfonic color Evans Blue (C.I.23860) in ratio 2:1. Lamellas are dark violet with light violet periphery. Obtained results speak in favor of high applicability of this method in light microscopy. In our opinion, this method can be recommended as one of methods for identification of elastic tissues.

Dipeptidyl peptidase IV is ecto-enzyme which is present on the cell surface of large number of cells and particularly of T-lymphocytes. It takes part in immune reactions and regulation of immune response, while antigen stimulation of cells increases percentage of expression of DPPIV on their cell surfaces. Since the immune response is different for chronic tonsillar disease of tonsilla palatine, idiopathic tonsillar hyperplasia ITH and recurrent tonsillitis RT, and considering the fact that DPPIV takes part in activation of T and B-lymphocytes stimulated by antigen, we presume that different activities of DPPIVexist in tonsillis and blood of persons diseased of different types of chronic tonsillitis, ITH and RT. The main goals of research are determination of activities of DPPIV on lymphocytes of tonsillas and serum of persons diseased of ITH and RT as well as histochemical and immunohistochemical determination of DPP IV localisation on lymphocytes of examed tonsillas. Two groups of patients are included: nineteen persons with ITH and thirteen persons with RT, while the control group was contained of thirty nine persons with diagnosis systematic lupus erythematosus (SLE) and fiftteen health persons. The research was done on 32 tonsillas created by tonsillectomia of persons with ITH and RT and 86 blood samples of all 86 persons. The results of research show histochemical localisation of enzyme activity of DPPIVin T-dependent zones of tonsilla and it is originating from activated T-lymphocytes. The enzyme activity of DPP IV on tonsillar lymphocytesis is higher in case of IHT compared with RT. By this research we conclude that deifferent activity of DPPIV on tonsillar lymphocytes for ITH and RTpresumes the difference of immuno response of tonsillas during these tonsillar diseases.

Anorectal abscesses and fistulas are problems that are commonly encountered together. Occasionally, their management can prove to be formidable, constantly challenging the clinical and surgical skills of the practicing surgeon. This article reviews the etiology, presentation, and treatment of anorectal abscesses and fistulas.

Parameters of anaerobic capacity were investigated in the group of 90 young subjects classed into diferent groups depent on their physical fitness. The group consisted of 30 wrestlers, 30 rowers and 30 students. Basic antropometric parameters were taken for each person and than Wingate anaerobic test was administrated. The aim of this investigation was to measure anaerobic parameters and determine weather there was a difference among sportsman and non sportsman. All parameters of Wingate test (anaerobic power, explosive power, anaerobic capacity) were significantly higher in the group of sportsman. (p0.05) Also, the highes values od anaerobic parameters were detected in the group of wrestlers, since this is typicaly anaerobic sport. The lower values were measured in the group of non sportsman, as expected.

We presented you six patients suffering from phlegmona periurethralis. It is mens' disease and, in the greatest degree, it appears at stenosis and urethra trauma. The commonest case of this disease are patients suffering from diabetes and those who carry catheter all the time. The appliance of a large doses of antibiotics and early incision with drainage can help patients to be cured. Urine derivation is very important and it must be like minimal cystostomy

The injuries of genital organs are commonest today regarding to the fact that there are different kinds of firearms and contact mines, although testicles are well protected in thigh area. In cases where we have maximum testicles injuries the conservative surgical treatment should be applied, considering the fact that testicles have great power of "restitucio ad integrum".

In well organized societies, accept of material and technical progress, planning of the family and existing of human resources is of great importance. Every civilized society is tancing care of human potential, planning and progress, eather to stimulate or control of natural increase are bringing the suitable demographics of standards. With better family planning asspecially motivatiy the couples to decide for reproduction and delivering of optimal namber of children in optimal period between deliveries and in optimal ages, brings to decreased mortality among new-borns, and also their mathers. 

All formsspondyloarthropaties are uniteded with HLA-B27 antigen's in 70-100% in dependences of form illneses and so ethenic and eugenic qualifications. Consider that some Gram-negative bacteriums can have place in pathogenesis of spondyloarthropaties. Explored are 24 patients(15 men and 9 women) with seronegative spondyloarthropaties. Absence of
bacteriums which besides other factors inculpate for pathogenesis seronegative spondyloarthropaties in explored materials,
points to need for proving their antigens at synovium woof or anti-bodies in serum toward specific antigens.

The complement system (complement) involves over 30 circulating and membrane-fixed proteins with an effector
role in the innate and humoral immunity. These proteins help the function of antibodies to protect the organism from foreign
molecules (antigens) which the term complement comes from. Soluble proteins of the complement system made mainly in
the liver and circulate in blood in an inactive form. The activation of complement may be initiated in three ways (classical,
alternative and lecitin pathway), and it is realized by sequential proteolysis of complement proteins (proenzymes) which
become emzymes with the proteolitic activity after cleavage. Products of the activatin of complement bind to the surface of
microbes or to the antibodies bound to antigens. An activated complement shows a number of biological effects, such as
lysis of an attached cell, opsonization, neutralization of viruses, inflammation, clearance of immune complexes etc. In contrast of microorganisms, human cells have a number of regulatory proteins which prevent the complement activation and in that way, they regulate its activity

Perinatal infection occurs as a consequence of the intrauterine (congenital) infection, intrapartal infection, or the
newborn infection in the course of its nursing. Congenital cytomegalovirus infection is a most frequent intrauterine infection. The prevalence of congenital CMV infection given by different authors varies significantly, but the majority of them
mention that it is in the range from 0.5 to 2.5 % of the number of the live-born. Significance of this disorder is underlined by
the fact that about 20% of intrauterine infected newborns have later serious problems in their development. The signs of fetal
impairments with congenital CMV infection can be noticed with about 10 % of the infected newborns at the very birth. The
mortality rate among these newborns is high even about 30 % of them exhibit heavy signs of the disease and die very soon
after the birth. Even with 70 % of the newborns with the symptoms of congenital CMV infection it is possible to notice at the
birth the presence of the symptomatology related to CNS, and this is of predicative significance for the later unfavorable
development of the infant. It should be pointed out that, in contrast to intrauterine infection, which provenly yields to fetal
impairments, there are no reliable data that would point to undesired outcomes of intra- or postpartal infections. The work
gives a survey of contemporary knowledge about cytomegalovirus infection in perinatology, as well as about the possibilities of its diagnosis and treatment.