Introduction: Solar or artificial UVA radiation play a major role in photoaging of the skin. Objective: The aim of our study was to determine the presence of morphological changes in the dermis of mouse skin exposed to UVA radiation, with or without the usage of photoprotective cream. Methods: This experiment involved the exposure of female BALBc mice, to solarium UVA lamps. The animals were divided into 2 experimental and 2 control groups. Before the radiation exposure, the hair on the backs of the experimental animals and one kontrol group was removed by shaving 3 times a week, and a half an hour before the exposure a broad spectrum photoprotective cream (UVA filter and SPF 50) was applied on half of the experimental animals. The animals were exposed to radiation 5 times a week, 2 hours a day (the dosage of 156 J/ cm2), for 16 weeks, up to a total radiation of 12500 J/cm2. Results: The presence of impaired collagen fibril network was the most pronounced in the experimental group of animals exposed to radiation without the usage of photoprotective cream (p<0.001). The presence of normal collagen fibril network was observed in the both control groups (p<0.001). The presence of incipient solar elastosis was observed in the experimental group of animals exposed to radiation without the usage of photoprotective cream. Conclusions: The results showed that the usage of a photoprotective cream provided an efficient protection against the effects of UVA radiation and dermal photoaging.

Determination of preoperative prostate-specific antigen (PSA) value is primary procedure in diagnosis of different pathological prostate changes (prostate cancer-PC, prostatic intraepithelial neoplasia-PIN and benign prostatic hyperplasia-BPH), followed by digital rectal examination and prostate biopsy as gold standard. Disadvantage of high sensitivity and low specificity of PSA testing in diagnosis of PC is a problem in clinical practice. Aim was to determine the diagnostic performance of PSA in diagnosis of PC, PIN and BPH. The study included 100 patients divided into three groups: 70 with PC, 20 with a PIN and 10 with BPH. Patients with PIN and BPH were control group. Preoperative PSA values were determined by Tandem-R, The patients were divided into subgroups by baseline PSA level as follows: 4-10, 11-20, 21-30, 31-40 and> 40. The definitive histopathological diagnosis was made on routine hematoxylineosin slides. The area under the receiver operating characteristic curve (ROC), sensitivity-SE and specificity-SP of each PSA level were evaluated for PC. Preoperative serum PSA levels in patients with PC (median-35.82 ng/ml, min-6 ng/ml, max-960.40 ng/ml) were significantly higher than with PIN (median-9.15 ng/ml, min-3.16 ng/ml, max-27.61 ng/ml) and BPH (median-8.68 ng/ml, min0.80 ng/ml, max-31.20 ng/ml). The best diagnostic characteristics of the PSA are on limit value 10 ng/ml (AUC=0.781, SE=92.9%; SP=63.3%; p<0,0001). PSA is of great help in diagnosis of advanced and initial form of PC. The chance of PC diagnosis was greater than that for other pathological changes when PSA level was higher than 10 ng/ml.