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Volume 53, Issue 4, 2025
Online ISSN: 2560-3310
ISSN: 0350-8773
Volume 53 , Issue 4, (2025)
Published: 30.06.2025.
Open Access
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Contents
01.12.2015.
Professional paper
Significance of echotomography in the diagnostic algorithm for acute pyelonephritis and glomerulonephritis
Introduction: In adults the diagnosis of acute pyelonephritis and glomerulonephritis is primarily based on clinical and laboratory-biochemical testing. In patients where the clinical picture atypical, even if a person does not respond to therapy resorts to radiographic examination. Echotomographic examination is unavoidable in the diagnostic algorithm. Objective: The aim of this study was to establish the individual echotomographic parameters, as well as to determine their diagnostic power in patients with acute infections (pyelonephritis and glomerulonephritis), and comparing them with the appropriate reference tests. Materials and methods: We performed a cross sectional study in the period from October 2014. until May 2015. It included 50 patients with acute inflammation of the kidney which was made echotomographic examination of the abdomen and pelvis, within the Department of Radiological Diagnostics KBC "Dragisa Mišović-Dedinje" in Belgrade. The echotomographic examination of the kidneys included testing of numerous parameters that could indicate the existence of an acute inflammation of the kidney. For the gold standard, we take the findings obtained by CT (computed tomography) imaging of the abdomen and pelvis, as well as histopathological findings obtained by fine needle bio-psy. Results: At 50 patients with acute inflammation of the upper urinary tract, 41 patients (82%) had acute pyelonephritis, and 9 (18%) had acute glomerulonephritis. In 70% of patients with acute pyelonephritis (29 people) were present enlargement of the kidney where the test sensitivity was 79.3% and specificity of 91.7%. The accuracy of the method was 82.9% when the monitored parameters: loss of central echo complex and cortico-medullary differentiation. The sensitivity of the test in which the observed thickening of the pelvic and ureteric wall was 65% and specificity of 90%. The analysis of the presence of calculus in renal parenchyma leads to the values of sensitivity test of 54.8% and specificity of 80%. Hypoechoic focus in the renal parenchyma, enlargement of the kidneys and loss corticomedullar limits are parameters who with great sensitivity and specificity suggest acute glomerulonephritis. Conclusion: On the basis of high values of sensitivity and specificity of the test survey estimates that ultrasound has a required place in the following diagnostics algorithm. The use of echotomography that offer the possibility of high resolutive views, as well as the wide availability and good reproducibility of the method, the low cost of inspection, in favor of the first exploration ultrasound examination. Multidetector CT scan and fine needle biopsy remains the method of choice for the definitive diagnosis.
Ivan Bogosavljević, M. Gašić, T. Filipović, P. Mandić, N. Đukić-Macut, M. Šaranović, S. Stajić
01.12.2014.
Professional paper
DERMATOGLIFI, ZNAČAJ I PRIMENA
Koža koja oblaže naše telo, ima ulogu školjke, tako da njen specifični “naborani” izgled odgovara grebenima.Tragovi koji ostaju na predmetima od “šara” na našim prstima zovu se otisci pratiju- dermatoglifi.Ove “šare” čine raznovrsne konfiguracije,karakteristične za svakog čoveka.Dermatoglifi su poligenetski determinisana svojstva,čiji izgled i brojnost određuju specifični geni.Obrasci kože su usko povezani sa strukturom nervnih završetaka u vrhovima prstiju.Što ukazuje na veoma preciznu povezanost u karakteristikama dizajna ljudskog mozga.Na osnovu toga možemo mnogo naučiti o nervnom sistemu, funkcijama, temperamentu i karakteru.Svedoci smo sve većeg značaja i primene dermatoglifa u svakodnevnom životu.
N. Djukic-Macut, T. Filipovic, P. Mandic
01.08.2015.
Professional paper
Comparative analysis of numerical density of ganglion cells with certain content of lipofuscin pigment in the parts of symphatetic trunk during the aging
The neurons of the sympathetic trunk as well as the other nerve cells undergo of many changes during life. The most striking of these morphological changes, during normal aging, is the accumulation of lipofuscin-filled vacuoles or neuromelanin. Considering that the pigment is a non-biodegradable and can not be removed by exocytosis, the process of its accumulation in cells is unavoidable. The role of lipofuscin and its impact on cell function is not quite clear. Some authors consider that pigment does not damage the function of the cell, unless it contains lipofuscin in large quantities, and then it mechanically prevents its function so that could lead to cell death. Since we found a very little data in the literature about using morphometric methods in accumulation of pigment in ganglion cells or quantified observed changes, we set that the aim of this study is to confirm the presence of pigment in ganglionar cells of the symphatetic trunk, when it occurs in grater extent, as well as dinamics of its accumulation (quantification of ganglionar cells without pigment, those with partial presence of pigment, and those that were complitely filled with pigment) by using numerical density. For morphometric analysis we used test system M42. To determine the numerical density of ganglionar cells we used a method for thick cuts by Floderus. We found that interneuronal accumulation of lipofuscin is directly correlated with the aging process.
T. Filipovic, P. Mandic, M. Filipovic, N. Djukic, S. Matejic, M. Saranovic
01.12.2013.
Professional paper
DNA identification of war and mass catastrophies victims
After the armed conflicts throughout the nineties of the twentieth century occurred in former Yugoslavia, identification of war victims is a challenging task. This paper gives a detailed description of exhumed remains identification process. One of the study objectives has been a comparison between DNA results and traditional forensic identification methods. This paper deals with the identification of human remains that were exhumed in Kosovo and Metohia in the period 2001-2012, belonging to Serbs and other non-Albanian ethnic communities (Montenegrins, Bosniaks, Roma, Gorani, and others), as well as much lower number of Albanians who were also killed during the war and post-war period.The experience of this process and the experience of other countries show that there is a need to organize appropriate services for identification of human remains in Serbia, capable to react effectively in case of mass disaster.
S. Matejic, M. Miletic, M. Milosevic, V. Jaksic, T. Filipovic, M. Saranovic, N. Deletic
01.01.2011.
Original scientific paper
MORPHOMETRIC ANALYSIS GANGLIONIC STRUCTURES OF MYENTERIC NERVOUS PLEXUS FORE WALL OF HUMAN STOMACH DURING PROCESS OF AGING
Aging as continous biological process, affect all organic systems. Aging is believed to affect the structure and function of the enteric nervous system. Prymary aim these studies is to verify changes in number of neurons in myenteric nervous plexus of human stomach within relation to process of aging. In the course of research played is analysis number of neurons in myenteric nervous plexus of anterior wall stomach. Analysis was performed within 30 tissue samples which are classified into three age groups: (from 20 to 44 years, from 45 to 64 years and over 65 to 84 year old). Played is histological processing and stained with HE, Cresyl-violet, AgNO3 and AChE methods. The morphometric measures operated by morphometric multipurpose test system M42. Finding results are statistically processed by Student-t-test and analysis of variance. Discernible is very heavy loss of neurons of myenteric plexus within the oldest group in relation to younger groups. Applied test show are having statistically notable variance between age groups. In percentage, the loss of neurons in the oldest group in relation to others was from 13,81% to 15,44%.
P. Mandić, T. Filipović, N. Đukić, V. Nedeljković
01.01.2010.
Original scientific paper
ANALYSIS OF CHANGES AND ACCUMULATION OF LIPOFUSCIN PIGMENT IN CELLS OF THORACIC GANGLIA OF SYMPATHETIC TRUNK DURING AGING
In the region of the thorax there are ten till twelve ganglia (ganglia thoracica); wich are white; triangle and irregular shape; rarely fusiform (spindle-shaped) or round. In the upper part of the thorax; ganglia are located in front of of the neck of ribs (collum costae); and in the middle part they are in front of head of ribs (caput costae). In the lower parts; they are near the vertebral bodies; therefore both of the trunks are slightly convergent. In this part; there are no branches which connected both sides of the sympathetic trunk. Lipofucin is yellow-brown; autofluorescent pigment; accumulated during aging in the lysosome of postmitotic cells; such as neurons.
T. Filipović, Z. Vitošević, P. Mandić, N. Đukić, M. Milisavljević, P. Stefanović
01.12.2009.
Original scientific paper
MORPHOMETRIC AND STEREOLOGICAL ANALYSIS GANGLION STRUCTURES OF MYENTERIC NERVE PLEXUS, TERMINALLY PART OF HUMAN LARGE INTESTINE
Myenteric nervous plexus is from rare importance for function of gastrointestinal tract. It perform one regulatory level autonomic nervous system which is situated within wall of digestive tract wherefore is directly exposed effect pathogenetic factors from extern ambiance. Aim of these study is that itself within terminal part of large intestine (sigmoid colon and rectum) particulary inquest myentric nervous plexus as part of autonomic nervous system. That itself describe shape and arrangement of ganglion structures, as well as, shape and arrangement of ganglion cells of myenteric nervous plexus. Within our researches we use totally 60 tissue samples of human sigmoid colon and rectum. From anterior wall those part of large intestine was taken 30 samples, also and same number of samples towards parts of posterior wall. Proximately past getting samples was determine their volume by using picnometre. Afterwards are made preparations coloured by He, Cresyl - violet and AgNO methods. Tissue samples are sliced within step - shared series of incisions with thickness slice by 7µm and 3 thickness grade by 50µm. Stereologycal analysis was perform by test system M42 which is calibrated on enlarge objective 40x. During analysis was determined absolute volume of ganglion structures of myenteric plexus and absolute number of ganglion cells inside of ganglion structures. Structures of myenteric nervous plexus being shown on longitudinal cross - section of tissues as flat, irregular ramify structure explicitly limited from surrounding smooth muscles. Immanent is upward trend absolute volume of ganglion structures and absolute number of ganglion cells goes from sigmoid colon according to ampullar part of rectum. Variance on level of significance by p<0,05 are present only if itself compare absolute volume of ganglion structures and absolute number of ganglion cells of myenteric plexus ampullar part of rectum and uppermost part of sigmoid colon. Test correlation shows of being presence high statistic significant (p<0,001) correlation between absolute volume of ganglions and absolute number of ganglion cells of myenteric nervous plexus.
P. Mandić, T. Filipović, N. Đukić, V. Nedeljković
01.01.2008.
Original scientific paper
EXHUMATION OFMASSIVE GRAVES AND SINGLE GRAVES ON THE TERRITORYOFKOSOVO AND METOHIJA(2001-2004)
Identification of victims during war and post war conflicts means for The Republic of Serbia the most important professional, scientific and ethical investigation. Before the process of identification forensics do the exhumation, very important part of process of which depends successful identification. On the Kosovo and Metohija, from 2001 till 2004. forensics did exhumation in some massive graves and in many single graves. Experiences of experts proove that the process of exhumation is very important becouse in that first phase collecting of evidence through forensic investigations have made “disappeared” victims “visible”, empowered survivors, corrected the historical records, and exposed cover-ups. Through forensic investigations including the identification of all injuries on the dead bodies, we faced with the marks and circumstances which followed the death under existing conditions on Kosovo and Metohija. Forensics promotes democratization and rule of law.
S. Matejić, M. Todorović, T. Filipović, M. Milošević, V. Jakšiċ
01.01.2007.
Original scientific paper
MACROSCOPIC VARIABILITY OF STUCTURES OF LOBULUS PARIETALIS AND PRECUNEUS
Traumas of CNS are more often and more complex than ever, also the influence of the factors of environment are more destructing, so all of them require detail knowledge of the morphology and functions of the nervous system. It is necessary for the faster and more accurate diagnosis in clinical medicine, and also it is necessary in preventing diseases of CNS. Modern neuroimaging procedures such as CT, MRI, PET, require precise knowledge of both morphologic variations of human brain , and the relation between morphology and function. In this kind of research the best solution is combination of descriptive and quantitative methods
N. Đukić-Macut, Z. Vitošević, T. Filipović, P. Mandić, M: Milisavljević, M. Šaranović
01.12.2007.
Original scientific paper
МОRPHOLOGICAL AND MORPHOMETRIC ANALYSIS OF ACCUMULATION THE LIPOFUSCIN PIGMENTIN GANGLIACELLS IN THE CERVICAL PARTOFSYMPATHETIC TRUNK IN THE PROCESS OFOLD AGE
Old age can be defined as a variety of changes in structure and function which begin at the stage of reproductive maturity, and that changes are manifested as a lesser ability of adaptation and lesser way of defending from the influence of outside and inside factors. Neurons of sympathetic trunk, as well as the other neuron cells, are changeable during the life. Neurobiological, old age begins at the time of birth, because the number of neurons is not increasing, it's only getting lower, which means that neurons are post mitotic cells. The process of old age is increased with some degenerative changes at some diseases and its hard to know what belongs to, so called, physiologic way of getting old and what is pathologic. Concentration of lipofuscin in cells (pigment of old age), is the parameter for physiologic old age of a person. Concentration is changeable, and it depends from the tissue or the organ, concentration is controlled by the internal regulatory factors and the factors of environment as the way of nutrition, physical activity, stress and hygienic factors.
T. Filipović, N. Stefanović, Z. Vitošević, M. Milisavljević, N. Đukić, P. Mandić, S. Matejić, M. Šaranović