After beeing liberated from the Turkish rule and establishing autonomy (self-gaverment ) serbian elite with big steaps theadet toward developing: social, economical and political lise in Serbia. About had time started to rise and developt official medical servis in principality of Serbia, under it's wing started to originate and medical biocemistry. The gool of this study is to point out and shear new light on istory of medical biohemistry in Serbia using adecvate literure and un publiehed archive documentacion