To show the most common causes of bacteriemia in hospitalized patients, as well as their sensitivity to antimicrobial agents. The research data were extracted from official reports produced by Microbiological Laboratory in Clinical Centre Kragujevac. Isolation and identification of the causes were performed with standard microbiological methodology. Sensitivity to antimicrobial medication was tested using disc diffusion. Interpretation and inhibition zones of antibiogram were done in accordance with guidelines of US Clinical and Laboratory Standards Institute (CLSI). A descriptive epidemiological approach was used in the research. The collected data were processed using the appropriate software application. Amongst the processed samples, a significant presence of Staphylococcus spp. was found (coagulase-negative 35,8% and coagulase positive 11,7%). Over 60% of Staphylococcus aureus isolates were resistant to meticillin. Enterococcus spp, isolated in 11,7% of all isolates, in more than 50% of cases was sensitive to ampicillin, with approximate isolate vancomycin sensitivity of 90%. The isolated Klebsiellà spp strains produced high degrees of Extended-Spectrum â-Lactamases (ESBL) and showed resistance to third-generation cephalosporins, aminoglycosides and ciprofloxacin, with 100% sensitivity to carbapenem. Pseudomonas spp and Acinetobacter spp were less represented (5.8% and 5.4%) but these neither pathogens were exempt to antimicrobial resistance.The research indicated that causes of hospital infections should be continuously monitored.